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Rabbit anti-CASP9/Caspase 9 Antibody, Affinity Purified

A305-252A
Bethyl Laboratories
ApplicationsWestern Blot
Product group Antibodies
TargetCASP9
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Overview

  • Supplier
    Bethyl Laboratories
  • Product Name
    Rabbit anti-CASP9/Caspase 9 Antibody, Affinity Purified
  • Delivery Days Customer
    9
  • Applications
    Western Blot
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1000 ug/ml
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID842
  • Target name
    CASP9
  • Target description
    caspase 9
  • Target synonyms
    APAF3; APAF-3; apoptotic protease activating factor 3; apoptotic protease MCH-6; caspase 9, apoptosis-related cysteine peptidase; caspase-9; ICE-LAP6; ICE-like apoptotic protease 6; MCH6; PPP1R56; protein phosphatase 1, regulatory subunit 56
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP55211
  • Protein Name
    Caspase-9
  • Storage Instruction
    2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of Caspase-9/CASP9 using anti-Caspase-9/CASP9 antibody (A00080-5). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human A549 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Caspase-9/CASP9 antigen affinity purified polyclonal antibody (Catalog # A00080-5) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Caspase-9/CASP9 at approximately 46KD. The expected band size for Caspase-9/CASP9 is at 46KD.
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