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ProductsBack/Rabbit anti-CD138 Recombinant Monoclonal Antibody [BLR323M]

Rabbit anti-CD138 Recombinant Monoclonal Antibody [BLR323M]

A700-323
Bethyl Laboratories
ApplicationsFlow Cytometry, ImmunoCytoChemistry, ImmunoHistoChemistry
Product group Antibodies
TargetSDC1
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Overview

  • Supplier
    Bethyl Laboratories
  • Product Name
    Rabbit anti-CD138 Recombinant Monoclonal Antibody [BLR323M]
  • Delivery Days Customer
    9
  • Applications
    Flow Cytometry, ImmunoCytoChemistry, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    BLR323M
  • Concentration
    500 ug/ml
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID6382
  • Target name
    SDC1
  • Target description
    syndecan 1
  • Target synonyms
    CD138, SDC, SYND1, syndecan, syndecan-1, CD138 antigen, heparan sulfate proteoglycan fibroblast growth factor receptor, syndecan proteoglycan 1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP18827
  • Protein Name
    Syndecan-1
  • Storage Instruction
    2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of Syndecan-1/SDC1 using anti-Syndecan-1/SDC1 antibody (A00991-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human placenta tissue lysate. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Syndecan-1/SDC1 antigen affinity purified polyclonal antibody (Catalog # A00991-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Syndecan-1/SDC1 at approximately 60KD. The expected band size for Syndecan-1/SDC1 is at 32KD.
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