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ProductsBack/Rabbit anti-CD48 Antibody, Affinity Purified

Rabbit anti-CD48 Antibody, Affinity Purified

A304-436A
Bethyl Laboratories
ApplicationsImmunoPrecipitation, Western Blot
Product group Antibodies
TargetCD48
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Overview

  • Supplier
    Bethyl Laboratories
  • Product Name
    Rabbit anti-CD48 Antibody, Affinity Purified
  • Delivery Days Customer
    9
  • Applications
    ImmunoPrecipitation, Western Blot
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1000 ug/ml
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID962
  • Target name
    CD48
  • Target description
    CD48 molecule
  • Target synonyms
    BCM1, BLAST, BLAST1, MEM-102, SLAMF2, hCD48, mCD48, CD48 antigen, B-lymphocyte activation marker BLAST-1, BCM1 surface antigen, CD48 antigen (B-cell membrane protein), SLAM family member 2, TCT.1, leukocyte antigen MEM-102, signaling lymphocytic activation molecule 2
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP09326
  • Protein Name
    CD48 antigen
  • Storage Instruction
    2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of CD48 using anti-CD48 antibody (A03281-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. lane 1: CEM whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD48 antigen affinity purified polyclonal antibody (Catalog # A03281-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CD48 at approximately 39KD. The expected band size for CD48 is at 28KD.
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