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ProductsBack/Rabbit anti-CRSP7 Antibody, Affinity Purified

Rabbit anti-CRSP7 Antibody, Affinity Purified

A302-370A
Bethyl Laboratories
ApplicationsImmunoPrecipitation
Product group Antibodies
TargetMED26
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Overview

  • Supplier
    Bethyl Laboratories
  • Product Name
    Rabbit anti-CRSP7 Antibody, Affinity Purified
  • Delivery Days Customer
    9
  • Applications
    ImmunoPrecipitation
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1000 ug/ml
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID9441
  • Target name
    MED26
  • Target description
    mediator complex subunit 26
  • Target synonyms
    CRSP7, CRSP70, mediator of RNA polymerase II transcription subunit 26, ARC70, CRSP complex subunit 7, activator-recruited cofactor 70 kDa component, cofactor required for Sp1 transcriptional activation subunit 7, cofactor required for Sp1 transcriptional activation, subunit 7 (70kD), cofactor required for Sp1 transcriptional activation, subunit 7, 70kDa, transcriptional coactivator CRSP70
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDO95402
  • Protein Name
    Mediator of RNA polymerase II transcription subunit 26
  • Storage Instruction
    2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of MED26 using anti-MED26 antibody (A09340-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human HepG2 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MED26 antigen affinity purified polyclonal antibody (Catalog # A09340-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MED26 at approximately 65 kDa. The expected band size for MED26 is at 65 kDa.
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IHC image of CSB-PA013659LA01HU diluted at 1:500 and staining in paraffin-embedded human testis tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
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