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Rabbit anti-DHX15 Antibody, Affinity Purified

A300-389A
Bethyl Laboratories
ApplicationsWestern Blot, ImmunoHistoChemistry
Product group Antibodies
TargetDHX15
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Overview

  • Supplier
    Bethyl Laboratories
  • Product Name
    Rabbit anti-DHX15 Antibody, Affinity Purified
  • Delivery Days Customer
    9
  • Applications
    Western Blot, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1000 ug/ml
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID1665
  • Target name
    DHX15
  • Target description
    DEAH-box helicase 15
  • Target synonyms
    DBP1, DDX15, PRP43, PRPF43, PrPp43p, hPrp43, ATP-dependent RNA helicase DHX15, ATP-dependent RNA helicase #46, DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 15, DEAD/H box-15, DEAH (Asp-Glu-Ala-His) box helicase 15, DEAH (Asp-Glu-Ala-His) box polypeptide 15, DEAH box protein 15, RNA helicase 2, pre-mRNA-splicing factor ATP-dependent RNA helicase DHX15, putative pre-mRNA-splicing factor ATP-dependent RNA helicase DHX15, splicing factor Prp43
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDO43143
  • Protein Name
    Pre-mRNA-splicing factor ATP-dependent RNA helicase DHX15
  • Storage Instruction
    2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of DHX15/prp43 using anti-DHX15/prp43 antibody (A08140-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human 293T whole cell lysates, Lane 3: human CACO-2 whole cell lysates, Lane 4: human HEL whole cell lysates, Lane 5: human RT4 whole cell lysates, Lane 6: human A549 whole cell lysates, Lane 7: human A431 whole cell lysates, Lane 8: human U251 whole cell lysates, Lane 9: rat C6 whole cell lysates, Lane 10: mouse thymus tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-DHX15/prp43 antigen affinity purified polyclonal antibody (Catalog # A08140-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for DHX15/prp43 at approximately 91 kDa. The expected band size for DHX15/prp43 is at 91 kDa.
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