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ProductsBack/Rabbit anti-E2-25K Antibody, Affinity Purified

Rabbit anti-E2-25K Antibody, Affinity Purified

A304-125A
Bethyl Laboratories
ApplicationsImmunoPrecipitation, Western Blot
Product group Antibodies
TargetUBE2K
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Overview

  • Supplier
    Bethyl Laboratories
  • Product Name
    Rabbit anti-E2-25K Antibody, Affinity Purified
  • Delivery Days Customer
    9
  • Applications
    ImmunoPrecipitation, Western Blot
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1000 ug/ml
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID3093
  • Target name
    UBE2K
  • Target description
    ubiquitin conjugating enzyme E2 K
  • Target synonyms
    E2-25K, HIP2, HYPG, LIG, UBC1, ubiquitin-conjugating enzyme E2 K, E2 ubiquitin-conjugating enzyme K, E2(25K), HIP-2, huntingtin-interacting protein 2, ubiquitin carrier protein, ubiquitin conjugating enzyme E2K, ubiquitin-conjugating enzyme E2(25K), ubiquitin-conjugating enzyme E2-25 KDA, ubiquitin-conjugating enzyme E2K (UBC1 homolog, yeast), ubiquitin-protein ligase
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP61086
  • Protein Name
    Ubiquitin-conjugating enzyme E2 K
  • Storage Instruction
    2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of HIP2/LIG/UBE2K using anti-HIP2/LIG/UBE2K antibody (A05895-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: monkey COS-7 whole cell lysates, Lane 2: human HEK293 whole cell lysates, Lane 3: rat brain tissue lysates, Lane 4: rat testis tissue lysates, Lane 5: mouse brain tissue lysates, Lane 6: mouse testis tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HIP2/LIG/UBE2K antigen affinity purified polyclonal antibody (Catalog # A05895-2) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for HIP2/LIG/UBE2K at approximately 22 kDa. The expected band size for HIP2/LIG/UBE2K is at 22 kDa.
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