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ProductsBack/Rabbit anti-FH/Fumarate Hydratase Antibody, Affinity Purified

Rabbit anti-FH/Fumarate Hydratase Antibody, Affinity Purified

A305-262A
Bethyl Laboratories
ApplicationsImmunoPrecipitation, Western Blot
Product group Antibodies
TargetFH
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Overview

  • Supplier
    Bethyl Laboratories
  • Product Name
    Rabbit anti-FH/Fumarate Hydratase Antibody, Affinity Purified
  • Delivery Days Customer
    9
  • Applications
    ImmunoPrecipitation, Western Blot
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1000 ug/ml
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID2271
  • Target name
    FH
  • Target description
    fumarate hydratase
  • Target synonyms
    FMRD, HLRCC, HsFH, LRCC, MCL, MCUL1, fumarate hydratase, mitochondrial, epididymis secretory sperm binding protein, fumarase
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP07954
  • Protein Name
    Fumarate hydratase, mitochondrial
  • Storage Instruction
    2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of FH using anti-FH antibody (A02097). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat lymphaden tissue lysates, Lane 2: rat small intestine tissue lysates, Lane 3: rat gaster tissue lysates, Lane 4: mouse kidney tissue lysates, Lane 5: mouse testis tissue lysates, Lane 6: mouse gaster tissue lysates, Lane 7: human K562 whole cell lysates, Lane 8: human U937 whole cell lysates, Lane 9:human HL-60 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FH antigen affinity purified polyclonal antibody (Catalog # A02097) at 0.5 ug/mL overnight at 4 then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for FH at approximately 49KD. The expected band size for FH is at 55KD.
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