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Rabbit anti-GRSF1 Antibody, Affinity Purified

A305-137A
Bethyl Laboratories
ApplicationsImmunoPrecipitation, Western Blot
Product group Antibodies
ReactivityHuman
TargetGRSF1

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Overview

  • Supplier
    Bethyl Laboratories
  • Product Name
    Rabbit anti-GRSF1 Antibody, Affinity Purified
  • Delivery Days Customer
    9
  • Applications
    ImmunoPrecipitation, Western Blot
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1000 ug/ml
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID2926
  • Target name
    GRSF1
  • Target description
    G-rich RNA sequence binding factor 1
  • Target synonyms
    G-rich sequence factor 1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ12849
  • Protein Name
    G-rich sequence factor 1
  • Reactivity
    Human
  • Storage Instruction
    2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of GRSF1 using anti-GRSF1 antibody (A07576-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human Jurkat whole cell lysates, Lane 3: human HEK293 whole cell lysates, Lane 4: human Raji whole cell lysates, Lane 5: human MCF-7 whole cell lysates, Lane 6: human HEPG2 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GRSF1 antigen affinity purified polyclonal antibody (Catalog # A07576-2) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for GRSF1 at approximately 53KD. The expected band size for GRSF1 is at 53KD.
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