More than 130 suppliers
ISO certified
In-house tech support
we Connect you
Bio-Connect
ProductsBack/Rabbit anti-HSP27 Antibody, Affinity Purified

Rabbit anti-HSP27 Antibody, Affinity Purified

A304-709A
Bethyl Laboratories
ApplicationsImmunoPrecipitation, Western Blot, ImmunoHistoChemistry
Product group Antibodies
TargetHSPB1
Sign in to order and to see your custom pricing.
Large volume orders?
Order with a bulk request
More than 130 suppliers
ISO certified
In-house tech support

Overview

  • Supplier
    Bethyl Laboratories
  • Product Name
    Rabbit anti-HSP27 Antibody, Affinity Purified
  • Delivery Days Customer
    9
  • Applications
    ImmunoPrecipitation, Western Blot, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1000 ug/ml
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID3315
  • Target name
    HSPB1
  • Target description
    heat shock protein family B (small) member 1
  • Target synonyms
    CMT2F, HEL-S-102, HMN2B, HMND3, HS.76067, HSP27, HSP28, Hsp25, SRP27, heat shock protein beta-1, 28 kDa heat shock protein, epididymis secretory protein Li 102, estrogen-regulated 24 kDa protein, heat shock 27 kDa protein, heat shock 27kD protein 1, heat shock 27kDa protein 1, heat shock protein family B member 1, stress-responsive protein 27
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP04792
  • Protein Name
    Heat shock protein beta-1
  • Storage Instruction
    2°C to 8°C
  • UNSPSC
    12352203

FAQ

Go to our frequently asked questions

Related products

Formalin-fixed and paraffin embedded rat brain labeled with Rabbit Anti-HSP27/HspB1/HSP25 Polyclonal Antibody, Unconjugated (bs-0730R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.
Product group Antibodies
References
Bioss
HSP27 Polyclonal AntibodyBS-0730R
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
TargetHSPB1
  • SizePrice
Product group Antibodies
LifeSpan BioSciences
HSPB1 / HSP27 Antibody (Preservative Free)LS-C149219
ApplicationsELISA
TargetHSPB1
  • SizePrice
Product group Antibodies
Cusabio
HSPB1 Monoclonal AntibodyCSB-MA080263
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
ReactivityHuman
TargetHSPB1
  • SizePrice
Product group Antibodies
MedChem Express
Phospho-Hsp27 (Ser82) Antibody (YA187, Rabbit)HY-P80465
TargetHSPB1
  • SizePrice
Product group Antibodies
References
GeneTex
HSP27 antibodyGTX101145
ApplicationsFlow Cytometry, ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
TargetHSPB1
  • SizePrice
Product group Antibodies
Absolute Antibody
Anti-HSPB1 [SAIC-23B-22]AB00313-1.1-BT
ApplicationsMass Spectrometry
TargetHSPB1
  • SizePrice
Anti-HSP27 Antibody
Product group Antibodies
Antibodies.com
Anti-HSP27 AntibodyA100672
ApplicationsELISA, ImmunoHistoChemistry
  • SizePrice
Product group Antibodies
Assay Genie
HSP27/HSPB1 AntibodyCAB0240
TargetHSPB1
  • SizePrice
Product group Antibodies
Biomatik
HSPB1 Polyclonal AntibodyCAC15125
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
TargetHSPB1
  • SizePrice
Figure 1. Western blot analysis of HSP27 using anti-HSP27 antibody (PB9237). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human A549 whole cell lysates, Lane 2: human CACO-2 whole cell lysates, Lane 3: human Hela whole cell lysates, Lane 4: human MCF-7 whole cell lysates, Lane 5: human HT1080 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HSP27 antigen affinity purified polyclonal antibody (Catalog # PB9237) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for HSP27 at approximately 27 kDa. The expected band size for HSP27 is at 27 kDa.
Product group Antibodies
Boster Bio
Anti-Hsp27/HSPB1 Antibody Picoband(r)PB9237-CARRIER-FREE
ApplicationsFlow Cytometry, ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen
TargetHSPB1
  • SizePrice