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ProductsBack/Rabbit anti Human ERCC1 (DNA excision repair protein)

Rabbit anti Human ERCC1 (DNA excision repair protein)

X2734P
Nordic-MUbio
ApplicationsWestern Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetERCC1
100 ug
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Overview

  • Supplier
    Nordic-MUbio
  • Product Name
    Rabbit anti Human ERCC1 (DNA excision repair protein)
  • Delivery Days Customer
    7
  • Application Supplier Note
    This antibody can be used for Western blotting (5-10 microg/ml) and immunohistochemistry on formalin/paraffin sections (5-10 microg/ml). Optimal concentration should be evaluated by serial dilutions.
  • Applications
    Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    Western Blotting;Immunohistochemistry (paraffin);Western Blotting;Immunohistochemistry (paraffin)
  • Category Supplier
    Primary antibodies
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Gene ID2067
  • Target name
    ERCC1
  • Target description
    ERCC excision repair 1, endonuclease non-catalytic subunit
  • Target synonyms
    COFS4; DNA excision repair protein ERCC-1; excision repair cross-complementation group 1; excision repair cross-complementing rodent repair deficiency, complementation group 1 (includes overlapping antisense sequence); RAD10; UV20
  • Host
    Rabbit
  • Protein IDP07992
  • Protein Name
    DNA excision repair protein ERCC-1
  • Scientific Description
    Excision Repair Cross Complementing protein-1; Nucleotide Excision Repair Protein
  • Shelf life instruction
    See expiration date on vial
  • Reactivity Supplier
    Human
  • Reactivity Supplier Note
    Synthetic peptide derived from human ERCC1 protein
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of ERCC1 using anti-ERCC1 antibody (A00388-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat stomach tissue lysates, Lane 2: mouse stomach tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ERCC1 antigen affinity purified polyclonal antibody (Catalog # A00388-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ERCC1 at approximately 33KD. The expected band size for ERCC1 is at 33KD.
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