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Anti-RTEL1 (N-term) Antibody

119-12836
RayBiotech
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetRTEL1
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Overview

  • Supplier
    RayBiotech
  • Product Name
    Anti-RTEL1 (N-term) Antibody
  • Delivery Days Customer
    4
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.5 mg/ml
  • Gene ID51750
  • Target name
    RTEL1
  • Target description
    regulator of telomere elongation helicase 1
  • Target synonyms
    C20orf41, DKCA4, DKCB5, NHL, PFBMFT3, RTEL, regulator of telomere elongation helicase 1, regulator of telomere length
  • Host
    Rabbit
  • Protein IDQ9NZ71
  • Protein Name
    Regulator of telomere elongation helicase 1
  • Scientific Description
    RTEL1 Rabbit anti-Human Polyclonal (N-Terminus) (Unconjugated) Antibody, (50 ug)
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of RTEL1 using anti-RTEL1 antibody (A01544-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat testis tissue lysates, Lane 2: rat PC-12 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RTEL1 antigen affinity purified polyclonal antibody (Catalog # A01544-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RTEL1 at approximately 134KD. The expected band size for RTEL1 is at 134KD.
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