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IHC-P analysis of human small intestine tissue using GTX85733 RTEL1 antibody. Antigen retrieval : Heat-induced antigen retrieval Dilution : 5 microg/ml
IHC-P analysis of human small intestine tissue using GTX85733 RTEL1 antibody. Antigen retrieval : Heat-induced antigen retrieval Dilution : 5 microg/ml
IHC-P analysis of human small intestine tissue using GTX85733 RTEL1 antibody. Antigen retrieval : Heat-induced antigen retrieval Dilution : 5 microg/ml

RTEL1 antibody

GTX85733
GeneTex
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
DownloadDatasheet
Product group Antibodies
TargetRTEL1
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Overview

  • Supplier
    GeneTex
  • Product Name
    RTEL1 antibody
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1 microg/ml. IHC-P: 5 microg/ml. ELISA: 1 microg/ml. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.5 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID51750
  • Target name
    RTEL1
  • Target description
    regulator of telomere elongation helicase 1
  • Target synonyms
    C20orf41; DKCA4; DKCB5; NHL; PFBMFT3; regulator of telomere elongation helicase 1; regulator of telomere length; RTEL
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9NZ71
  • Protein Name
    Regulator of telomere elongation helicase 1
  • Scientific Description
    This gene encodes a DNA helicase which functions in the stability, protection and elongation of telomeres and interacts with proteins in the shelterin complex known to protect telomeres during DNA replication. Mutations in this gene have been associated with dyskeratosis congenita and Hoyerall-Hreidarsson syndrome. Read-through transcription of this gene into the neighboring downstream gene, which encodes tumor necrosis factor receptor superfamily, member 6b, generates a non-coding transcript. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Sep 2013]
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of RTEL1 using anti-RTEL1 antibody (A01544-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat testis tissue lysates, Lane 2: rat PC-12 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RTEL1 antigen affinity purified polyclonal antibody (Catalog # A01544-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RTEL1 at approximately 134KD. The expected band size for RTEL1 is at 134KD.
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