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ProductsBack/Rabbit anti Human Sphingosine 1 Phosphate Receptor 1 (EDG-1) CT

Rabbit anti Human Sphingosine 1 Phosphate Receptor 1 (EDG-1) CT

X1093P
Nordic-MUbio
ApplicationsWestern Blot
Product group Antibodies
TargetS1PR1
100 ug
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Overview

  • Supplier
    Nordic-MUbio
  • Product Name
    Rabbit anti Human Sphingosine 1 Phosphate Receptor 1 (EDG-1) CT
  • Delivery Days Customer
    7
  • Application Supplier Note
    Detects EDG1 receptors at concentration of 5-10 microg/ml by Western blot using a human EDG1 receptor transfected cell line. Detects an approximately 44 kDa band in RH7777 cells transfected with full length human EDG1. Due to low expression of EDG receptors, we recommend use of Pierce Femto Signal substrate for western blot development. Optimal concentration should be evaluated by serial dilutions.
  • Applications
    Western Blot
  • Applications Supplier
    Western Blotting;Western Blotting
  • Category Supplier
    Primary antibodies
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID1901
  • Target name
    S1PR1
  • Target description
    sphingosine-1-phosphate receptor 1
  • Target synonyms
    CD363; CHEDG1; D1S3362; ECGF1; EDG1; EDG-1; endothelial differentiation G-protein coupled receptor 1; endothelial differentiation, sphingolipid G-protein-coupled receptor, 1; S1P receptor 1; S1P receptor Edg-1; S1P1; sphingosine 1-phosphate receptor 1; sphingosine 1-phosphate receptor EDG1; sphingosine 1-phosphate receptor Edg-1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP21453
  • Protein Name
    Sphingosine 1-phosphate receptor 1
  • Scientific Description
    Endothelial cell differentiation gene 1 C-terminal
  • Shelf life instruction
    See expiration date on vial
  • Reactivity Supplier
    Human;Mouse;Rat
  • Reactivity Supplier Note
    Synthetic peptide derived from the C-terminal of the EDG-1 receptor
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of EDG1 using anti-EDG1 antibody (M01502). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human U-87MG whole cell lysates, Lane 3: human SH-SY5Y whole cell lysates, Lane 4: human U251 whole cell lysates, Lane 5: rat brain tissue lysates, Lane 6: rat C6 whole cell lysates, Lane 7: mouse brain tissue lysates, Lane 8: mouse Neuro-2a whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-EDG1 antigen affinity purified monoclonal antibody (Catalog # M01502) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:500 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for EDG1 at approximately 43 kDa. The expected band size for EDG1 is at 43 kDa.
Product group Antibodies
References
Boster Bio
Anti-EDG1 S1PR1 Rabbit Monoclonal AntibodyM01502
ApplicationsWestern Blot
TargetS1PR1
  • SizePrice
Product group Antibodies
RayBiotech
Anti-S1PR1 Antibody144-12935
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Biomatik
S1PR1 Polyclonal AntibodyCAC13921
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Assay Genie
EDG1/HEXIM1 AntibodyCAB12935
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Western Blot analysis of HT-29 cells using EDG-1 Polyclonal Antibody
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Cusabio
S1PR1 AntibodyCSB-PA002247
ApplicationsImmunoFluorescence, Western Blot, ELISA
ReactivityHuman, Mouse, Rat
TargetS1PR1
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Product group Antibodies
LifeSpan BioSciences
S1PR1 / EDG1 / S1P1 AntibodyLS-C402837
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TargetS1PR1
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Anti-S1PR1 Antibody
Product group Antibodies
Antibodies.com
Anti-S1PR1 AntibodyA43229
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Immunofluorescent staining of human cell line U-251 MG shows localization to vesicles.
Product group Antibodies
Atlas Antibodies
Anti-S1PR1 AntibodyHPA075568
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ReactivityHuman
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WB analysis of rat Brain (Lane 1) and Neuro- 2a (Lane 2) lysate using GTX11424 EDG1 antibody. Dilution : 2 microg/ml
Product group Antibodies
GeneTex
EDG1 antibodyGTX11424
ApplicationsFlow Cytometry, ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen
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Rat heart lysates probed with S1P1 Polyclonal Antibody, unconjugated (bs-7112R) at 1:300 overnight at 4°C followed by a conjugated secondary antibody at 1:10000 for 90 minutes at 37°C.
Product group Antibodies
References
Bioss Antibodies
EDG1 Polyclonal AntibodyBS-7112R
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TargetS1PR1
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