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Rabbit anti Human SURF1

X1562P
Nordic-MUbio
ApplicationsWestern Blot
Product group Antibodies
TargetSURF1
100 ug
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Overview

  • Supplier
    Nordic-MUbio
  • Product Name
    Rabbit anti Human SURF1
  • Delivery Days Customer
    7
  • Application Supplier Note
    Antibody can be used for Western blot (1-5 microg/ml). Optimal concentration should be evaluated by serial dilutions.
  • Applications
    Western Blot
  • Applications Supplier
    Western Blotting;Western Blotting
  • Category Supplier
    Primary antibodies
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID6834
  • Target name
    SURF1
  • Target description
    SURF1 cytochrome c oxidase assembly factor
  • Target synonyms
    CMT4K; MC4DN1; SHY1; surfeit 1; surfeit locus protein 1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ15526
  • Protein Name
    Surfeit locus protein 1
  • Scientific Description
    Human Ssu72
  • Shelf life instruction
    See expiration date on vial
  • Reactivity Supplier
    Human
  • Reactivity Supplier Note
    Full length recombinant human Ssu72 protein
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of SURF1 using anti-SURF1 antibody (A03678-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human 293T whole cell lysates, Lane 3: monkey COS-7 whole cell lysates, Lane 4: rat heart tissue lysates, Lane 5: rat kidney tissue lysates, Lane 6: mouse heart tissue lysates, Lane 7: mouse kidney tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SURF1 antigen affinity purified polyclonal antibody (Catalog # A03678-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SURF1 at approximately 33 kDa. The expected band size for SURF1 is at 33 kDa.
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