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ProductsBack/Rabbit anti-IL-12B Recombinant Monoclonal Antibody [BLR322M]

Rabbit anti-IL-12B Recombinant Monoclonal Antibody [BLR322M]

A700-322
Bethyl Laboratories
ApplicationsImmunoPrecipitation, Western Blot
Product group Antibodies
TargetIL12B
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Overview

  • Supplier
    Bethyl Laboratories
  • Product Name
    Rabbit anti-IL-12B Recombinant Monoclonal Antibody [BLR322M]
  • Delivery Days Customer
    9
  • Applications
    ImmunoPrecipitation, Western Blot
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    BLR322M
  • Concentration
    1000 ug/ml
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID3593
  • Target name
    IL12B
  • Target description
    interleukin 12B
  • Target synonyms
    CLMF, CLMF2, IL-12B, IMD28, IMD29, NKSF, NKSF2, interleukin-12 subunit beta, CLMF p40, IL-12 subunit p40, IL12, subunit p40, NK cell stimulatory factor chain 2, cytotoxic lymphocyte maturation factor 40 kDa subunit, interleukin 12, p40, interleukin 12B (natural killer cell stimulatory factor 2, cytotoxic lymphocyte maturation factor 2, p40), interleukin-12 beta chain, natural killer cell stimulatory factor, 40 kD subunit
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP29460
  • Protein Name
    Interleukin-12 subunit beta
  • Storage Instruction
    2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of IL12B using anti-IL12B antibody (A01152-4). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: mouse NIH3T3 cell lysates, Lane 2: human A549 cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IL12B antigen affinity purified polyclonal antibody (Catalog # A01152-4) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for IL12B at approximately 37KD. The expected band size for IL12B is at 37KD.
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