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ProductsBack/Goat anti-IL12B / IL12p40

Goat anti-IL12B / IL12p40

EB07862
Everest Biotech
ApplicationsWestern Blot, ELISA
Product group Antibodies
TargetIL12B
100 ug
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Overview

  • Supplier
    Everest Biotech
  • Product Name
    Goat anti-IL12B / IL12p40 Antibody
  • Delivery Days Customer
    5
  • Applications
    Western Blot, ELISA
  • Applications Supplier
    Pep-ELISA, WB
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.5 mg/ml
  • Gene ID3593
  • Target name
    IL12B
  • Target description
    interleukin 12B
  • Target synonyms
    CLMF; CLMF p40; CLMF2; cytotoxic lymphocyte maturation factor 40 kDa subunit; IL-12 subunit p40; IL12, subunit p40; IL-12B; IMD28; IMD29; interleukin 12, p40; interleukin 12B (natural killer cell stimulatory factor 2, cytotoxic lymphocyte maturation factor 2, p40); interleukin-12 beta chain; interleukin-12 subunit beta; natural killer cell stimulatory factor, 40 kD subunit; NK cell stimulatory factor chain 2; NKSF; NKSF2
  • Host
    Goat
  • Scientific Description
    RefSeq number(s): NP_002178.2. Purification: Antigen affinity purified. Names and symbols: IL12B; interleukin 12B (natural killer cell stimulatory factor 2, cytotoxic lymphocyte maturation factor 2, p40) ; CLMF; CLMF2; IL-12B; NKSF; NKSF2; IL12; subunit p40; cytotoxic lymphocyte maturation factor 2, p40; interleukin 12, p40; interleukin 12B; in
  • Reactivity Supplier
    Human
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Mouse spleen lysates probed with Rabbit Anti-IL-12 beta Polyclonal Antibody, Unconjugated (bs-10641R) at 1:300 overnight at 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:5000 for 90 min at 37˚C.
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Figure 1. Western blot analysis of IL12B using anti-IL12B antibody (A01152-4). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: mouse NIH3T3 cell lysates, Lane 2: human A549 cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IL12B antigen affinity purified polyclonal antibody (Catalog # A01152-4) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for IL12B at approximately 37KD. The expected band size for IL12B is at 37KD.
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References
Everest Biotech
Goat anti-Il12b / Il12p40 (mouse)EB07863
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