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Rabbit anti-IP3R1 Antibody, Affinity Purified

A302-157A
Bethyl Laboratories
ApplicationsImmunoPrecipitation, Western Blot
Product group Antibodies
TargetITPR1
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Overview

  • Supplier
    Bethyl Laboratories
  • Product Name
    Rabbit anti-IP3R1 Antibody, Affinity Purified
  • Delivery Days Customer
    9
  • Applications
    ImmunoPrecipitation, Western Blot
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    200 ug/ml
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID3708
  • Target name
    ITPR1
  • Target description
    inositol 1,4,5-trisphosphate receptor type 1
  • Target synonyms
    ACV, CLA4, INSP3R1, IP3R, IP3R1, PPP1R94, SCA15, SCA16, SCA29, inositol 1,4,5-trisphosphate-gated calcium channel ITPR1, IP3 receptor, IP3R 1, inositol 1,4,5 trisphosphate receptor, inositol 1,4,5-triphosphate receptor, type 1, protein phosphatase 1, regulatory subunit 94, type 1 InsP3 receptor, type 1 inositol 1,4,5-trisphosphate receptor
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ14643
  • Protein Name
    Inositol 1,4,5-trisphosphate receptor type 1
  • Storage Instruction
    2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of IP3 receptor using anti-IP3 receptor antibody (PB9225). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: rat brain tissue lysates, Lane 3: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IP3 receptor antigen affinity purified polyclonal antibody (Catalog # PB9225) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for IP3 receptor at approximately 290 kDa. The expected band size for IP3 receptor is at 314 kDa.
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