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Rabbit anti-LARG Antibody, Affinity Purified

A301-959A
Bethyl Laboratories
ApplicationsWestern Blot
Product group Antibodies
TargetARHGEF12
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Overview

  • Supplier
    Bethyl Laboratories
  • Product Name
    Rabbit anti-LARG Antibody, Affinity Purified
  • Delivery Days Customer
    9
  • Applications
    Western Blot
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1000 ug/ml
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID23365
  • Target name
    ARHGEF12
  • Target description
    Rho guanine nucleotide exchange factor 12
  • Target synonyms
    LARG; leukemia-associated rho guanine nucleotide exchange factor; leukemia-associated RhoGEF; PRO2792; Rho guanine nucleotide exchange factor (GEF) 12; rho guanine nucleotide exchange factor 12
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9NZN5
  • Protein Name
    Rho guanine nucleotide exchange factor 12
  • Storage Instruction
    2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of LARG/ARHGEF12 using anti-LARG/ARHGEF12 antibody (A06802-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HEL whole cell lysates, Lane 2: human K562 whole cell lysates, Lane 3: rat brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-LARG/ARHGEF12 antigen affinity purified polyclonal antibody (Catalog # A06802-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The si2093gnal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for LARG/ARHGEF12 at approximately 220 kDa. The expected band size for LARG/ARHGEF12 is at 173 kDa.
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