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Rabbit anti-MBD4 IHC Antibody, Affinity Purified

IHC-00329
Bethyl Laboratories
ApplicationsImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Primate
TargetMBD4

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Overview

  • Supplier
    Bethyl Laboratories
  • Product Name
    Rabbit anti-MBD4 IHC Antibody, Affinity Purified
  • Delivery Days Customer
    9
  • Applications
    ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    250 ug/ml
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID8930
  • Target name
    MBD4
  • Target description
    methyl-CpG binding domain 4, DNA glycosylase
  • Target synonyms
    MED1, TPDS2, UVM1, methyl-CpG-binding domain protein 4, 3,N(4)-ethenocytosine glycosylase, G/5-fluorouracil mismatch glycosylase with biphasic kinetics, G/T mismatch glycosylase, G/U mismatch glycosylase, methyl-CpG binding domain protein 4, methyl-CpG-binding endonuclease 1, methyl-CpG-binding protein MBD4, mismatch-specific DNA N-glycosylase, putative methyl-CpG binding protein
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDO95243
  • Protein Name
    Methyl-CpG-binding domain protein 4
  • Reactivity
    Human, Primate
  • Storage Instruction
    2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of MBD4/MED1 using anti-MBD4/MED1 antibody (A03462). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human A549 whole cell lysates, Lane 2: human Jurkat whole cell lysates, Lane 3: human SK-OV-3 whole cell lysates, Lane 4: human Raji whole cell lysates, Lane 5: human hela whole cell lysates, Lane 6: human A431 whole cell lysates, Lane 7: human MDA-MB-453 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MBD4/MED1 antigen affinity purified polyclonal antibody (Catalog # A03462) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MBD4/MED1 at approximately 66KD. The expected band size for MBD4/MED1 is at 66KD.
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