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ProductsBack/Rabbit anti-MIF Recombinant Monoclonal Antibody [BLR141J]

Rabbit anti-MIF Recombinant Monoclonal Antibody [BLR141J]

A700-141
Bethyl Laboratories
ApplicationsFlow Cytometry, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
Product group Antibodies
TargetMIF
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Overview

  • Supplier
    Bethyl Laboratories
  • Product Name
    Rabbit anti-MIF Recombinant Monoclonal Antibody [BLR141J]
  • Delivery Days Customer
    9
  • Applications
    Flow Cytometry, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    BLR141J
  • Concentration
    50 ug/ml
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID4282
  • Target name
    MIF
  • Target description
    macrophage migration inhibitory factor
  • Target synonyms
    GIF, GLIF, MMIF, macrophage migration inhibitory factor, L-dopachrome isomerase, L-dopachrome tautomerase, epididymis secretory sperm binding protein, macrophage migration inhibitory factor (glycosylation-inhibiting factor), phenylpyruvate tautomerase
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP14174
  • Protein Name
    Macrophage migration inhibitory factor
  • Storage Instruction
    2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of MIF using anti-MIF antibody (PB9274). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Jurkat whole cell lysates, Lane 2: human K562 whole cell lysates, Lane 3: human Hela whole cell lysates, Lane 4: human HepG2 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MIF antigen affinity purified polyclonal antibody (Catalog # PB9274) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MIF at approximately 12 kDa. The expected band size for MIF is at 12 kDa.
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