Sample (30 μg of whole cell lysate) A: Molt-4 (GTX27912) 15% SDS PAGE GTX101162 diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
![Various tissue extracts (50 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with MIF antibody [N1C3] (GTX101162) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.](https://www.genetex.com/upload/website/prouct_img/normal/GTX101162/GTX101162_39834_20171006_WB_M_tissue_w_23060100_152.webp "Various tissue extracts (50 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with MIF antibody [N1C3] (GTX101162) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.")
![MIF antibody [N1C3] detects MIF protein at cytoplasm in mouse brain by immunohistochemical analysis. Sample: Paraffin-embedded mouse brain. MIF antibody [N1C3] (GTX101162) diluted at 1:500.
Antigen Retrieval: Trilogy? (EDTA based, pH 8.0) buffer, 15min](https://www.genetex.com/upload/website/prouct_img/normal/GTX101162/GTX101162_39834_20170929_IHC-P_M_w_23060100_227.webp "MIF antibody [N1C3] detects MIF protein at cytoplasm in mouse brain by immunohistochemical analysis. Sample: Paraffin-embedded mouse brain. MIF antibody [N1C3] (GTX101162) diluted at 1:500.
Antigen Retrieval: Trilogy? (EDTA based, pH 8.0) buffer, 15min")
![MIF antibody [N1C3] detects MIF protein at cytoplasm in rat brain by immunohistochemical analysis. Sample: Paraffin-embedded rat brain. MIF antibody [N1C3] (GTX101162) diluted at 1:500.
Antigen Retrieval: Trilogy? (EDTA based, pH 8.0) buffer, 15min](https://www.genetex.com/upload/website/prouct_img/normal/GTX101162/GTX101162_39834_20170929_IHC-P_R_w_23060100_213.webp "MIF antibody [N1C3] detects MIF protein at cytoplasm in rat brain by immunohistochemical analysis. Sample: Paraffin-embedded rat brain. MIF antibody [N1C3] (GTX101162) diluted at 1:500.
Antigen Retrieval: Trilogy? (EDTA based, pH 8.0) buffer, 15min")
![Various whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with MIF antibody [N1C3] (GTX101162) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX101162/GTX101162_39834_20240531_WB_24060619_699.webp "Various whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with MIF antibody [N1C3] (GTX101162) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.")
![Various whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with MIF antibody [N1C3] (GTX101162) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.](https://www.genetex.com/upload/website/prouct_img/normal/GTX101162/GTX101162_45329_20240412_WB_TPM_watermark_24072519_195.webp "Various whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with MIF antibody [N1C3] (GTX101162) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.")
Sample (30 μg of whole cell lysate) A: Molt-4 (GTX27912) 15% SDS PAGE GTX101162 diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
MIF antibody [N1C3]
GTX101162
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetMIF
Overview
- SupplierGeneTex
- Product NameMIF antibody [N1C3]
- Delivery Days Customer9
- Application Supplier NoteWB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. ELISA: 1:1000-1:10000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration0.1 mg/ml
- ConjugateUnconjugated
- Gene ID4282
- Target nameMIF
- Target descriptionmacrophage migration inhibitory factor
- Target synonymsGIF, GLIF, MMIF, macrophage migration inhibitory factor, L-dopachrome isomerase, L-dopachrome tautomerase, epididymis secretory sperm binding protein, macrophage migration inhibitory factor (glycosylation-inhibiting factor), phenylpyruvate tautomerase
- HostRabbit
- IsotypeIgG
- Protein IDP14174
- Protein NameMacrophage migration inhibitory factor
- Scientific DescriptionThis gene encodes a lymphokine involved in cell-mediated immunity, immunoregulation, and inflammation. It plays a role in the regulation of macrophage function in host defense through the suppression of anti-inflammatory effects of glucocorticoids. This lymphokine and the JAB1 protein form a complex in the cytosol near the peripheral plasma membrane, which may indicate an additional role in integrin signaling pathways. [provided by RefSeq]
- ReactivityHuman, Mouse, Rat
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203
References
- Murata R, Watanabe H, Nosaki H, et al. Long-Acting Thioredoxin Ameliorates Doxorubicin-Induced Cardiomyopathy via Its Anti-Oxidative and Anti-Inflammatory Action. Pharmaceutics. 2022,14(3). doi: 10.3390/pharmaceutics14030562Read this paper
- Chen LL, Lee MH, Chang CL, et al. Suppression of Inflammatory and Fibrotic Signals by Cinnamon (Cinnamomum cassia) and Cinnamaldehyde in Cyclophosphamide-Induced Overactive Bladder in Mice. Evid Based Complement Alternat Med. 2021,2021:5205759. doi: 10.1155/2021/5205759Read this paper
- Yulizal OK, Lelo A, Ilyas S, et al. The effect of snakehead fish extract supplementation to first-line eradication regimen on macrophage migration inhibitory factor (MIF) expression in rats induced by Helicobacter pylori infection. J Adv Vet Anim Res. 2020,7(2):209-217. doi: 10.5455/javar.2020.g411Read this paper
- Movila A, Ishii T, Albassam A, et al. Macrophage Migration Inhibitory Factor (MIF) Supports Homing of Osteoclast Precursors to Peripheral Osteolytic Lesions. J Bone Miner Res. 2016,31(9):1688-700. doi: 10.1002/jbmr.2854Read this paper
Datasheet
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![Western blot analysis of GeneTex's IgY fraction of Chicken-anti-Human MIF polyclonal antibody shows the detection of 100 μg of recombinant MIF present in a lysate. Similar detection of MIF will occur when human serum is analyzed. In lane 1 no reaction is observed in the control whereas lane 2 shows a single band at 12.3 kDa. A 4-20% gradient gel was used to separate the proteins by SDS-PAGE. The protein was transferred to nitro-cellulose using standard methods. After blocking the membrane was probed with the primary antibody for 1 h at room temperature followed by washes and reaction with a 1:5,000 dilution of IRDye800 conjugated Gt-a-Chicken Rabbit IgG [H&L] for 1 h at room temperature. LICOR's OdysseyR Infrared Imaging System was used to scan and process the image. Other detection systems will yield similar results.](https://www.genetex.com/upload/website/prouct_img/normal/GTX48478/GTX48478_20160330_WB_w_23060823_846.webp)
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