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Rabbit anti-MMACHC Antibody, Affinity Purified

A304-541A
Bethyl Laboratories
ApplicationsWestern Blot
Product group Antibodies
ReactivityBovine, Human
TargetMMACHC

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Overview

  • Supplier
    Bethyl Laboratories
  • Product Name
    Rabbit anti-MMACHC Antibody, Affinity Purified
  • Delivery Days Customer
    9
  • Applications
    Western Blot
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1000 ug/ml
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID25974
  • Target name
    MMACHC
  • Target description
    metabolism of cobalamin associated C
  • Target synonyms
    cblC, cyanocobalamin reductase / alkylcobalamin dealkylase, alkylcobalamin:glutathione S-alkyltransferase, cyanocobalamin reductase (cyanide-eliminating), methylmalonic aciduria (cobalamin deficiency) cblC type, with homocystinuria, methylmalonic aciduria and homocystinuria type C protein
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9Y4U1
  • Protein Name
    Cyanocobalamin reductase / alkylcobalamin dealkylase
  • Reactivity
    Bovine, Human
  • Storage Instruction
    2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of MMACHC using anti-MMACHC antibody (A03398-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human K562 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MMACHC antigen affinity purified polyclonal antibody (Catalog # A03398-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MMACHC at approximately 32 kDa. The expected band size for MMACHC is at 32 kDa.
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