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ProductsBack/Rabbit anti-NEDD-9/CASL/HEF1 Recombinant Monoclonal Antibody [BLR248L]

Rabbit anti-NEDD-9/CASL/HEF1 Recombinant Monoclonal Antibody [BLR248L]

A700-248
Bethyl Laboratories
ApplicationsImmunoPrecipitation, Western Blot
Product group Antibodies
TargetNEDD9
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Overview

  • Supplier
    Bethyl Laboratories
  • Product Name
    Rabbit anti-NEDD-9/CASL/HEF1 Recombinant Monoclonal Antibody [BLR248L]
  • Delivery Days Customer
    9
  • Applications
    ImmunoPrecipitation, Western Blot
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    BLR248L
  • Concentration
    1000 ug/ml
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID4739
  • Target name
    NEDD9
  • Target description
    neural precursor cell expressed, developmentally down-regulated 9
  • Target synonyms
    CAS-L, CAS2, CASL, CASS2, HEF1, enhancer of filamentation 1, Cas scaffolding protein family member 2, Crk-associated substrate related protein Cas-L, Enhancer of filamentation 1 p55, cas-like docking, neural precursor cell expressed developmentally down-regulated protein 9, p130Cas-related protein, renal carcinoma antigen NY-REN-12
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ14511
  • Protein Name
    Enhancer of filamentation 1
  • Storage Instruction
    2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of HEF1 using anti-HEF1 antibody (PB9289). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human A549 whole cell lysates, Lane 2: human Daudi whole cell lysates, Lane 3: monkey COS-7 whole cell lysates, Lane 4: mouse lung tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HEF1 antigen affinity purified polyclonal antibody (Catalog # PB9289) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for HEF1 at approximately 93-105 kDa. The expected band size for HEF1 is at 93 kDa.
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