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Rabbit anti-NUP107 Antibody, Affinity Purified

A301-787A
Bethyl Laboratories
ApplicationsImmunoPrecipitation, Western Blot
Product group Antibodies
TargetNUP107
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Overview

  • Supplier
    Bethyl Laboratories
  • Product Name
    Rabbit anti-NUP107 Antibody, Affinity Purified
  • Delivery Days Customer
    9
  • Applications
    ImmunoPrecipitation, Western Blot
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    200 ug/ml
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID57122
  • Target name
    NUP107
  • Target description
    nucleoporin 107
  • Target synonyms
    GAMOS7, NPHS11, NUP84, ODG6, ODG6; GAMOS7, nuclear pore complex protein Nup107, nucleoporin 107kDa
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP57740
  • Protein Name
    Nuclear pore complex protein Nup107
  • Storage Instruction
    2°C to 8°C
  • UNSPSC
    12352203

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Western Blot Positive WB detected in: Hela whole cell lysate, HEK293 whole cell lysate, NIH/3T3 whole cell lysate, PC-3 whole cell lysate All lanes: NUP107 antibody at 3.4microg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 107, 104, 80 kDa Observed band size: 107 kDa
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Figure 1. Western blot analysis of NUP107 using anti-NUP107 antibody (A03724-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Colo320 whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: human 293T whole cell lysates, Lane 4: human Hela whole cell lysates, Lane 5: human PC-3 whole cell lysates, Lane 6: human K562 whole cell lysates, Lane 7: human HEL whole cell lysates, Lane 8: rat PC-12 whole cell lysates, Lane 9: mouse testis tissue lysates, Lane 10: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NUP107 antigen affinity purified polyclonal antibody (Catalog # A03724-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NUP107 at approximately 106 kDa. The expected band size for NUP107 is at 106 kDa.
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