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Rabbit anti-PARN Antibody, Affinity Purified

A303-562A
Bethyl Laboratories
ApplicationsImmunoPrecipitation, Western Blot, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse, Primate
TargetPARN

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Overview

  • Supplier
    Bethyl Laboratories
  • Product Name
    Rabbit anti-PARN Antibody, Affinity Purified
  • Delivery Days Customer
    9
  • Applications
    ImmunoPrecipitation, Western Blot, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1000 ug/ml
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID5073
  • Target name
    PARN
  • Target description
    poly(A)-specific ribonuclease
  • Target synonyms
    DAN, DKCB6, PFBMFT4, poly(A)-specific ribonuclease PARN, deadenylating nuclease, deadenylation nuclease, polyadenylate-specific ribonuclease
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDO95453
  • Protein Name
    Poly(A)-specific ribonuclease PARN
  • Reactivity
    Human, Mouse, Primate
  • Storage Instruction
    2°C to 8°C
  • UNSPSC
    12352203

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Western blot All lanes: PARN antibody at 2microg/ml Lane 1: Jurkat whole cell lysate Lane 2: A431 whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 74, 67, 68, 53 kDa Observed band size: 74 kDa
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Figure 1. Western blot analysis of PARN using anti-PARN antibody (A01501-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human placenta tissue lysates, Lane 3: human COLO-320 whole cell lysates, Lane 4: human HepG2 whole cell lysates, Lane 5: human PANC-1 whole cell lysates, Lane 6: human SGC-7901 whole cell lysates, Lane 7: human MDA-MB-231 whole cell lysates, Lane 8: rat kidney tissue lysates, Lane 9: mouse heart tissue lysates, Lane 10: mouse kidney tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PARN antigen affinity purified polyclonal antibody (Catalog # A01501-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PARN at approximately 78KD. The expected band size for PARN is at 73KD.
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