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Rabbit anti-PML Antibody, Affinity Purified

A301-167A
Bethyl Laboratories
ApplicationsImmunoPrecipitation, Western Blot, ImmunoHistoChemistry
Product group Antibodies
TargetPML
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Overview

  • Supplier
    Bethyl Laboratories
  • Product Name
    Rabbit anti-PML Antibody, Affinity Purified
  • Delivery Days Customer
    9
  • Applications
    ImmunoPrecipitation, Western Blot, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    200 ug/ml
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID5371
  • Target name
    PML
  • Target description
    PML nuclear body scaffold
  • Target synonyms
    MYL, PP8675, RNF71, TRIM19, protein PML, E3 SUMO-protein ligase PML, PML/RARA fusion, RING finger protein 71, RING-type E3 SUMO transferase PML, probable transcription factor PML, promyelocytic leukemia protein, promyelocytic leukemia, inducer of, tripartite motif protein TRIM19, tripartite motif-containing protein 19
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP29590
  • Protein Name
    Protein PML
  • Storage Instruction
    2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of PML using anti-PML antibody (PB10084). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human HEK293 whole cell lysates, Lane 3: human U87 whole cell lysates, Lane 4: human A431 whole cell lysates, Lane 5: human K562 whole cell lysates. red to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PML antigen affinity purified polyclonal antibody (Catalog # PB10084) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PML at approximately 90-160 kDa. The expected band size for PML is at 98 kDa.
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