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Rabbit anti-PNPase Antibody, Affinity Purified

A303-917A
Bethyl Laboratories
ApplicationsImmunoPrecipitation, Western Blot
Product group Antibodies
TargetPNPT1
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Overview

  • Supplier
    Bethyl Laboratories
  • Product Name
    Rabbit anti-PNPase Antibody, Affinity Purified
  • Delivery Days Customer
    9
  • Applications
    ImmunoPrecipitation, Western Blot
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1000 ug/ml
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID87178
  • Target name
    PNPT1
  • Target description
    polyribonucleotide nucleotidyltransferase 1
  • Target synonyms
    COXPD13, DFNB70, OLD35, PNPASE, SCA25, old-35, polyribonucleotide nucleotidyltransferase 1, mitochondrial, 3'-5' RNA exonuclease OLD35, PNPase 1, PNPase old-35, polynucleotide phosphorylase 1, polynucleotide phosphorylase-like protein
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ8TCS8
  • Protein Name
    Polyribonucleotide nucleotidyltransferase 1, mitochondrial
  • Storage Instruction
    2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of PNPT1 using anti-PNPT1 antibody (A05460-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat brain tissue lysates, Lane 2: rat kidney tissue lysates, Lane 3: rat C6 whole cell lysates, Lane 4: mouse brain tissue lysates, Lane 5: mouse kidney tissue lysates, Lane 6: mouse Neuro-2a whole cell lysates, Lane 7: human K562 whole cell lysates, Lane 8: human THP-1 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PNPT1 antigen affinity purified polyclonal antibody (Catalog # A05460-1) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PNPT1 at approximately 86KD. The expected band size for PNPT1 is at 86KD.
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