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Rabbit anti-PolA1 Antibody, Affinity Purified

A302-850A
Bethyl Laboratories
ApplicationsImmunoPrecipitation, Western Blot
Product group Antibodies
ReactivityHuman, Mouse
TargetPOLA1

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Overview

  • Supplier
    Bethyl Laboratories
  • Product Name
    Rabbit anti-PolA1 Antibody, Affinity Purified
  • Delivery Days Customer
    9
  • Applications
    ImmunoPrecipitation, Western Blot
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    200 ug/ml
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID5422
  • Target name
    POLA1
  • Target description
    DNA polymerase alpha 1, catalytic subunit
  • Target synonyms
    NSX, PDR, POLA, VEODS, p180, DNA polymerase alpha catalytic subunit, DNA polymerase alpha catalytic subunit p180, polymerase (DNA directed), alpha 1, catalytic subunit, polymerase (DNA) alpha 1, catalytic subunit, polymerase (DNA-directed), alpha (70kD)
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP09884
  • Protein Name
    DNA polymerase alpha catalytic subunit
  • Reactivity
    Human, Mouse
  • Storage Instruction
    2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of POLA1 using anti-POLA1 antibody (A04421-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human HEK293 whole cell lysates, Lane 3: human Jurkat whole cell lysates, Lane 4: human K562 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-POLA1 antigen affinity purified polyclonal antibody (Catalog # A04421-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for POLA1 at approximately 180 kDa. The expected band size for POLA1 is at 180 kDa.
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