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Rabbit anti-PolH Antibody, Affinity Purified

A301-230A
Bethyl Laboratories
ApplicationsImmunoPrecipitation
Product group Antibodies
TargetPOLH
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Overview

  • Supplier
    Bethyl Laboratories
  • Product Name
    Rabbit anti-PolH Antibody, Affinity Purified
  • Delivery Days Customer
    9
  • Applications
    ImmunoPrecipitation
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1000 ug/ml
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID5429
  • Target name
    POLH
  • Target description
    DNA polymerase eta
  • Target synonyms
    RAD30, RAD30A, XP-V, XPV, DNA polymerase eta, DNA polymerase eta transcript, RAD30 homolog A, polymerase (DNA directed), eta, polymerase (DNA) eta, xeroderma pigmentosum variant type protein
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9Y253
  • Protein Name
    DNA polymerase eta
  • Storage Instruction
    2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of POLH using anti-POLH antibody (PB9813). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. Lane 1: Rat Liver Tissue Lysate at 50ug, Lane 2: HELA Whole Cell Lysate at 40ug, Lane 3: SW620 Whole Cell Lysate at 40ug. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-POLH antigen affinity purified polyclonal antibody (Catalog # PB9813) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for POLH at approximately 78 kDa. The expected band size for POLH is at 78 kDa.
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