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ProductsBack/Rabbit anti-POLR1D Antibody, Affinity Purified

Rabbit anti-POLR1D Antibody, Affinity Purified

A304-847A
Bethyl Laboratories
ApplicationsWestern Blot
Product group Antibodies
TargetPOLR1D
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Overview

  • Supplier
    Bethyl Laboratories
  • Product Name
    Rabbit anti-POLR1D Antibody, Affinity Purified
  • Delivery Days Customer
    9
  • Applications
    Western Blot
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1000 ug/ml
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID51082
  • Target name
    POLR1D
  • Target description
    RNA polymerase I and III subunit D
  • Target synonyms
    AC19, POLR1C, RPA16, RPA9, RPAC2, RPC16, RPO1-3, TCS2, DNA-directed RNA polymerases I and III subunit RPAC2, Protein POLR1D, DNA-directed RNA polymerase I subunit D, RNA polymerase I subunit D, RNA polymerases I and III subunit AC2, polymerase (RNA) I polypeptide D, 16kDa, polymerase (RNA) I subunit D
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP0DPB6
  • Protein Name
    DNA-directed RNA polymerases I and III subunit RPAC2
  • Storage Instruction
    2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of POLR1D using anti-POLR1D antibody (A07808-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human Jurkat whole cell lysates, Lane 3: human HepG2 whole cell lysates, Lane 4: human HEL whole cell lysates, Lane 5: rat lung tissue lysates, Lane 6: mouse lung tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-POLR1D antigen affinity purified polyclonal antibody (Catalog # A07808-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for POLR1D at approximately 20 kDa. The expected band size for POLR1D is at 15 kDa.
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