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Rabbit anti-PRPF8 Antibody, Affinity Purified

A303-920A
Bethyl Laboratories
ApplicationsWestern Blot, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse
TargetPRPF8

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Overview

  • Supplier
    Bethyl Laboratories
  • Product Name
    Rabbit anti-PRPF8 Antibody, Affinity Purified
  • Delivery Days Customer
    9
  • Applications
    Western Blot, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1000 ug/ml
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID10594
  • Target name
    PRPF8
  • Target description
    pre-mRNA processing factor 8
  • Target synonyms
    HPRP8, PRP8, PRPC8, RP13, SNRNP220, pre-mRNA-processing-splicing factor 8, 220 kDa U5 snRNP-specific protein, PRP8 homolog, PRP8 pre-mRNA processing factor 8 homolog, U5 snRNP-specific protein (220 kD), ortholog of S. cerevisiae Prp8p, apoptosis-regulated protein 1, apoptosis-regulated protein 2, p220, precursor mRNA processing protein, splicing factor Prp8
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ6P2Q9
  • Protein Name
    Pre-mRNA-processing-splicing factor 8
  • Reactivity
    Human, Mouse
  • Storage Instruction
    2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of PRPF8 using anti-PRPF8 antibody (A02878-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human SH-SY5Y whole cell lysates, Lane 3: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PRPF8 antigen affinity purified polyclonal antibody (Catalog # A02878-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PRPF8 at approximately 250 kDa. The expected band size for PRPF8 is at 274 kDa.
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Immunofluorescence staining of Hela cells with CSB-PA744036LA01HU at 1:166, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
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Sample (30 ug of whole cell lysate) A: Raji 5% SDS PAGE GTX108046 diluted at 1:500
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