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Rabbit anti-PSMA1 Antibody, Affinity Purified

A303-845A
Bethyl Laboratories
ApplicationsWestern Blot, ImmunoHistoChemistry
Product group Antibodies
TargetPSMA1
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Overview

  • Supplier
    Bethyl Laboratories
  • Product Name
    Rabbit anti-PSMA1 Antibody, Affinity Purified
  • Delivery Days Customer
    9
  • Applications
    Western Blot, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1000 ug/ml
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID5682
  • Target name
    PSMA1
  • Target description
    proteasome 20S subunit alpha 1
  • Target synonyms
    HC2, HEL-S-275, NU, PROS30, proteasome subunit alpha type-1, 30 kDa prosomal protein, PROS-30, alpha-6, epididymis secretory protein Li 275, macropain subunit C2, macropain subunit nu, multicatalytic endopeptidase complex subunit C2, proteasome (prosome, macropain) subunit, alpha type, 1, proteasome component C2, proteasome nu chain, proteasome subunit alpha 1, proteasome subunit alpha 6, proteasome subunit nu, proteasome subunit, alpha-type, 1, protein P30-33K, testicular tissue protein Li 150
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP25786
  • Protein Name
    Proteasome subunit alpha type-1
  • Storage Instruction
    2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of PSMA1 using anti-PSMA1 antibody (A07591-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human MCF-7 whole cell lysates, Lane 2: human PC-3 whole cell lysates, Lane 3: human HepG2 whole cell lysates, Lane 4: human Jurkat whole cell lysates, Lane 5: rat brain tissue lysates, Lane 6: rat liver tissue lysates, Lane 7: mouse brain tissue lysates, Lane 8: mouse liver tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PSMA1 antigen affinity purified polyclonal antibody (Catalog # A07591-1) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PSMA1 at approximately 33 kDa. The expected band size for PSMA1 is at 29-33 kDa.
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