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ProductsBack/Rabbit anti Rat Kv1.4 Potassium Channel (NT)

Rabbit anti Rat Kv1.4 Potassium Channel (NT)

X1500P
Nordic-MUbio
ApplicationsWestern Blot
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetKCNA4
100 ug
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Overview

  • Supplier
    Nordic-MUbio
  • Product Name
    Rabbit anti Rat Kv1.4 Potassium Channel (NT)
  • Delivery Days Customer
    7
  • Application Supplier Note
    This antibody can be used for Western blotting (5-10 microg/ml). Optimal concentration should be evaluated by serial dilutions.
  • Applications
    Western Blot
  • Applications Supplier
    Western Blotting;Western Blotting
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID3739
  • Target name
    KCNA4
  • Target description
    potassium voltage-gated channel subfamily A member 4
  • Target synonyms
    HBK4, HK1, HPCN2, HUKII, KCNA4L, KCNA8, KV1.4, MCIDDS, PCN2, potassium voltage-gated channel subfamily A member 4, cardiac potassium channel, fetal skeletal muscle potassium channel, potassium channel 2, potassium channel, voltage gated shaker related subfamily A, member 4, rapidly inactivating potassium channel, shaker-related potassium channel Kv1.4, type A potassium channel, voltage-gated K(+) channel HuKII, voltage-gated potassium channel HBK4, voltage-gated potassium channel HK1, voltage-gated potassium channel subunit Kv1.4
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP22459
  • Protein Name
    Potassium voltage-gated channel subfamily A member 4
  • Scientific Description
    Voltage-Gated Potassium Channel, Kv1.4 isoform
  • Shelf life instruction
    See expiration date on vial
  • Reactivity
    Human, Mouse, Rat
  • Reactivity Supplier
    Human;Mouse;Rat
  • Reactivity Supplier Note
    Synthetic peptide derived from the N-terminus of the rat Kv1.4 potassium channel conjugated to KLH
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of Kv1.4 using anti-Kv1.4 antibody (PB9807). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 40 ug of sample under reducing conditions. Lane 1: HELA Whole Cell Lysate, Lane 2: COLO320 Whole Cell Lysate, Lane 3: HT1080 Whole Cell Lysate, Lane 4: PANC Whole Cell Lysate. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Kv1.4 antigen affinity purified polyclonal antibody (Catalog # PB9807) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Kv1.4 at approximately 73 kDa. The expected band size for Kv1.4 is at 73 kDa.
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