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ProductsBack/Rabbit anti Rat Kv1.5 Potassium Channel

Rabbit anti Rat Kv1.5 Potassium Channel

X1501P
Nordic-MUbio
ApplicationsWestern Blot
Product group Antibodies
TargetKCNA5
100 ug
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Overview

  • Supplier
    Nordic-MUbio
  • Product Name
    Rabbit anti Rat Kv1.5 Potassium Channel
  • Delivery Days Customer
    7
  • Application Supplier Note
    This antibody can be used for Western blotting (5-10 microg/ml). Optimal concentration should be evaluated by serial dilutions.
  • Applications
    Western Blot
  • Applications Supplier
    Western Blotting
  • Category Supplier
    Primary antibodies
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID3741
  • Target name
    KCNA5
  • Target description
    potassium voltage-gated channel subfamily A member 5
  • Target synonyms
    ATFB7; cardiac potassium channel; HCK1; HK2; HPCN1; insulinoma and islet potassium channel; KV1.5; PCN1; potassium channel 1; potassium channel, voltage gated shaker related subfamily A, member 5; potassium voltage-gated channel subfamily A member 5; potassium voltage-gated channel, shaker-related subfamily, member 5; voltage-gated potassium channel HK2; voltage-gated potassium channel protein Kv1.5; voltage-gated potassium channel subunit Kv1.5
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP22460
  • Protein Name
    Potassium voltage-gated channel subfamily A member 5
  • Scientific Description
    Voltage-Gated Potassium Channel, Kv1.5 isoform
  • Shelf life instruction
    See expiration date on vial
  • Source
    Rabbits were immunized with a synthetic peptide derived from the rat Kv1.5 potassium channel conjugated to KLH
  • Reactivity Supplier
    Human;Mouse;Rat
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of KCNA5 using anti-KCNA5 antibody (PB9651). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. Lane 1: 293T Whole Cell Lysate at 40ug, Lane 2: A549 Whole Cell Lysate at 40ug, Lane 3: PANC Whole Cell Lysate at 40ug. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-KCNA5 antigen affinity purified polyclonal antibody (Catalog # PB9651) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for KCNA5 at approximately 67 kDa. The expected band size for KCNA5 is at 67 kDa.
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