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ProductsBack/Rabbit anti-Ribosomal Protein S8/RPS8 Antibody, Affinity Purified

Rabbit anti-Ribosomal Protein S8/RPS8 Antibody, Affinity Purified

A305-016A
Bethyl Laboratories
ApplicationsImmunoPrecipitation, Western Blot
Product group Antibodies
ReactivityBovine, Human, Mouse, Rat
TargetRPS8

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Overview

  • Supplier
    Bethyl Laboratories
  • Product Name
    Rabbit anti-Ribosomal Protein S8/RPS8 Antibody, Affinity Purified
  • Delivery Days Customer
    9
  • Applications
    ImmunoPrecipitation, Western Blot
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1000 ug/ml
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID6202
  • Target name
    RPS8
  • Target description
    ribosomal protein S8
  • Target synonyms
    S8, eS8, small ribosomal subunit protein eS8, 40S ribosomal protein S8, OK/SW-cl.83
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP62241
  • Protein Name
    Small ribosomal subunit protein eS8
  • Reactivity
    Bovine, Human, Mouse, Rat
  • Storage Instruction
    2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of RPS8 using anti-RPS8 antibody (M07839). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human THP-1 whole cell lysates, Lane 3: human K562 whole cell lysates, Lane 4: human HEL whole cell lysates, Lane 5: rat PC-12 whole cell lysates, Lane 6: rat C6 whole cell lysates, Lane 7: mouse ANA-1 whole cell lysates, Lane 8: mouse RAW264.7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RPS8 antigen affinity purified monoclonal antibody (M07839) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:500 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RPS8 at approximately 26 kDa. The expected band size for RPS8 is at 24 kDa.
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