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Rabbit anti-RMP Antibody, Affinity Purified

A301-163A
Bethyl Laboratories
ApplicationsImmunoPrecipitation, Western Blot
Product group Antibodies
ReactivityHuman
TargetURI1

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Overview

  • Supplier
    Bethyl Laboratories
  • Product Name
    Rabbit anti-RMP Antibody, Affinity Purified
  • Delivery Days Customer
    9
  • Applications
    ImmunoPrecipitation, Western Blot
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    200 ug/ml
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID8725
  • Target name
    URI1
  • Target description
    URI1 prefoldin like chaperone
  • Target synonyms
    C19orf2, NNX3, PPP1R19, RMP, URI, unconventional prefoldin RPB5 interactor 1, RNA polymerase II subunit 5-mediating protein, RPB5-mediating protein, protein phosphatase 1, regulatory subunit 19
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDO94763
  • Protein Name
    Unconventional prefoldin RPB5 interactor 1
  • Reactivity
    Human
  • Storage Instruction
    2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of C19orf2/URI1 using anti-C19orf2/URI1 antibody (A05245-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human U-87MG whole cell lysates, Lane 2: human K562 whole cell lysates, Lane 3: human Daudi whole cell lysates, Lane 4: human HEL1 whole cell lysates, Lane 5: mouse ovary tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-C19orf2/URI1 antigen affinity purified polyclonal antibody (Catalog # A05245-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for C19orf2/URI1 at approximately 80 kDa. The expected band size for C19orf2/URI1 is at 60 kDa.
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