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ProductsBack/Rabbit anti-SF3a120/SAP114 Antibody, Affinity Purified

Rabbit anti-SF3a120/SAP114 Antibody, Affinity Purified

A301-603A
Bethyl Laboratories
ApplicationsImmunoPrecipitation, Western Blot, ImmunoHistoChemistry
Product group Antibodies
ReactivityBovine, Human, Mouse
TargetSF3A1

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Overview

  • Supplier
    Bethyl Laboratories
  • Product Name
    Rabbit anti-SF3a120/SAP114 Antibody, Affinity Purified
  • Delivery Days Customer
    9
  • Applications
    ImmunoPrecipitation, Western Blot, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    200 ug/ml
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID10291
  • Target name
    SF3A1
  • Target description
    splicing factor 3a subunit 1
  • Target synonyms
    PRP21, PRPF21, SAP114, SF3A120, splicing factor 3A subunit 1, SAP 114, pre-mRNA processing 21, pre-mRNA splicing factor SF3a (120 kDa subunit), spliceosome-associated protein 114, splicing factor 3 subunit 1, splicing factor 3a, subunit 1, 120kD, splicing factor 3a, subunit 1, 120kDa
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ15459
  • Protein Name
    Splicing factor 3A subunit 1
  • Reactivity
    Bovine, Human, Mouse
  • Storage Instruction
    2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of SF3A1 using anti-SF3A1 antibody (A05483-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human Jurkat whole cell lysates, Lane 3: human K562 whole cell lysates, Lane 4: human 293T whole cell lysates, Lane 5: human U251 whole cell lysates, Lane 6: human Daudi whole cell lysates, Lane 7: human MOLT-4 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SF3A1 antigen affinity purified polyclonal antibody (Catalog # A05483-1) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SF3A1 at approximately 120-130 kDa. The expected band size for SF3A1 is at 89 kDa.
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