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ProductsBack/Rabbit anti-SF3b145/SAP145 Antibody, Affinity Purified

Rabbit anti-SF3b145/SAP145 Antibody, Affinity Purified

A301-605A
Bethyl Laboratories
ApplicationsImmunoPrecipitation, Western Blot, ImmunoHistoChemistry
Product group Antibodies
TargetSF3B2
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Overview

  • Supplier
    Bethyl Laboratories
  • Product Name
    Rabbit anti-SF3b145/SAP145 Antibody, Affinity Purified
  • Delivery Days Customer
    9
  • Applications
    ImmunoPrecipitation, Western Blot, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    200 ug/ml
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID10992
  • Target name
    SF3B2
  • Target description
    splicing factor 3b subunit 2
  • Target synonyms
    CFM, Cus1, HFM, SAP145, SF3B145, SF3b1, SF3b150, splicing factor 3B subunit 2, SAP 145, pre-mRNA splicing factor SF3b 145 kDa subunit, spliceosome associated protein 145, splicing factor 3b, subunit 2, 145kD, splicing factor 3b, subunit 2, 145kDa
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ13435
  • Protein Name
    Splicing factor 3B subunit 2
  • Storage Instruction
    2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of SF3B2 using anti-SF3B2 antibody (A07047-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Jurkat whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human 293T whole cell lysates, Lane 4: human K562 whole cell lysates, Lane 5: rat brain tissue lysates, Lane 6: rat RH-35 whole cell lysates, Lane 7: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SF3B2 antigen affinity purified polyclonal antibody (Catalog # A07047-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SF3B2 at approximately 120 kDa. The expected band size for SF3B2 is at 100 kDa.
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