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Rabbit anti-SF3B4 Antibody, Affinity Purified

A303-950A
Bethyl Laboratories
ApplicationsImmunoPrecipitation, Western Blot, ImmunoHistoChemistry
Product group Antibodies
TargetSF3B4
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Overview

  • Supplier
    Bethyl Laboratories
  • Product Name
    Rabbit anti-SF3B4 Antibody, Affinity Purified
  • Delivery Days Customer
    9
  • Applications
    ImmunoPrecipitation, Western Blot, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1000 ug/ml
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID10262
  • Target name
    SF3B4
  • Target description
    splicing factor 3b subunit 4
  • Target synonyms
    AFD1, Hsh49, SAP49, SF3b49, splicing factor 3B subunit 4, SAP 49, SF3b50, pre-mRNA-splicing factor SF3b 49 kDa subunit, spliceosomal protein, spliceosome-associated protein (U2 snRNP), spliceosome-associated protein 49, splicing factor 3b, subunit 4, 49kD, splicing factor 3b, subunit 4, 49kDa
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ15427
  • Protein Name
    Splicing factor 3B subunit 4
  • Storage Instruction
    2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of SF3B4 using anti-SF3B4 antibody (A06414-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human MCF-7 whole cell lysates, Lane 2: human U2OS whole cell lysates, Lane 3: human HGC-27 whole cell lysates, Lane 4: human GES-1 whole cell lysates, Lane 5: rat brain tissue lysates, Lane 6: rat stomach tissue lysates, Lane 7: mouse brain tissue lysates, Lane 8: mouse stomach tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SF3B4 antigen affinity purified polyclonal antibody (Catalog # A06414-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SF3B4 at approximately 44 kDa. The expected band size for SF3B4 is at 44 kDa.
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