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Rabbit anti-SGT1 Antibody, Affinity Purified

A302-775A
Bethyl Laboratories
ApplicationsImmunoPrecipitation, Western Blot
Product group Antibodies
TargetECD
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Overview

  • Supplier
    Bethyl Laboratories
  • Product Name
    Rabbit anti-SGT1 Antibody, Affinity Purified
  • Delivery Days Customer
    9
  • Applications
    ImmunoPrecipitation, Western Blot
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    200 ug/ml
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID11319
  • Target name
    ECD
  • Target description
    ecdysoneless cell cycle regulator
  • Target synonyms
    GCR2, HSGT1, SGT1, protein ecdysoneless homolog, ecdysoneless homolog, human suppressor of GCR two, protein SGT1, suppressor of GCR2, suppressor of S. cerevisiae gcr2
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDO95905
  • Protein Name
    Protein ecdysoneless homolog
  • Storage Instruction
    2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of SGT1/ECD using anti-SGT1/ECD antibody (A00567-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: mouse Ana-1 whole cell lysates, Lane 2: human HL-60 whole cell lysates, Lane 3: human U20S whole cell lysates, Lane 4: human U-87MG whole cell lysates, Lane 5: human K562 whole cell lysates, Lane 6: human HepG2 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SGT1/ECD antigen affinity purified polyclonal antibody (Catalog # A00567-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SGT1/ECD at approximately 73 kDa. The expected band size for SGT1/ECD is at 73 kDa.
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