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Rabbit anti-SMRT Antibody, Affinity Purified

A301-147A
Bethyl Laboratories
ApplicationsImmunoPrecipitation, Western Blot
Product group Antibodies
TargetNCOR2
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Overview

  • Supplier
    Bethyl Laboratories
  • Product Name
    Rabbit anti-SMRT Antibody, Affinity Purified
  • Delivery Days Customer
    9
  • Applications
    ImmunoPrecipitation, Western Blot
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    200 ug/ml
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID9612
  • Target name
    NCOR2
  • Target description
    nuclear receptor corepressor 2
  • Target synonyms
    CTG26, N-CoR2, SMAP270, SMRT, SMRTE, SMRTE-tau, TNRC14, TRAC, TRAC-1, TRAC1, nuclear receptor corepressor 2, CTG repeat protein 26, T3 receptor-associating factor, silencing mediator for retinoid and thyroid hormone receptors, thyroid-, retinoic-acid-receptor-associated corepressor
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9Y618
  • Protein Name
    Nuclear receptor corepressor 2
  • Storage Instruction
    2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of NCOR2 using anti-NCOR2 antibody (A01412-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human U20S whole cell lysates, Lane 2: human U251 whole cell lysates, Lane 3: human Hela whole cell lysates, Lane 4: human PC-3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NCOR2 antigen affinity purified polyclonal antibody (Catalog # A01412-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NCOR2 at approximately 275 kDa. The expected band size for NCOR2 is at 275 kDa.
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