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Rabbit anti-SRC1 Antibody, Affinity Purified

A300-343A
Bethyl Laboratories
ApplicationsImmunoPrecipitation, Western Blot, ImmunoHistoChemistry
Product group Antibodies
TargetNCOA1
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Overview

  • Supplier
    Bethyl Laboratories
  • Product Name
    Rabbit anti-SRC1 Antibody, Affinity Purified
  • Delivery Days Customer
    9
  • Applications
    ImmunoPrecipitation, Western Blot, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1000 ug/ml
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID8648
  • Target name
    NCOA1
  • Target description
    nuclear receptor coactivator 1
  • Target synonyms
    F-SRC-1, KAT13A, RIP160, SRC1, bHLHe42, bHLHe74, nuclear receptor coactivator 1, Hin-2 protein, class E basic helix-loop-helix protein 74, renal carcinoma antigen NY-REN-52, steroid receptor coactivator-1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ15788
  • Protein Name
    Nuclear receptor coactivator 1
  • Storage Instruction
    2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of KAT13A/SRC1 using anti-KAT13A/SRC1 antibody (RP1056). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: SK-OV-3 whole cell lysates, Lane 2: SGC-7901 whole cell lysates, Lane 3: Jurkat whole cell lysates, Lane 4: rat kidney tissue lysates, Lane 5: NIH/3T3 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-KAT13A/SRC1 antigen affinity purified polyclonal antibody (Catalog # RP1056) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for KAT13A/SRC1 at approximately 157KD. The expected band size for KAT13A/SRC1 is at 157KD.
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