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Rabbit anti-SUPT5H Antibody, Affinity Purified

ORB1529480
Biorbyt
ApplicationsWestern Blot, ImmunoHistoChemistry
Product group Antibodies
TargetSUPT5H
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Overview

  • Supplier
    Biorbyt
  • Product Name
    Rabbit anti-SUPT5H Antibody, Affinity Purified
  • Delivery Days Customer
    10
  • Application Supplier Note
    Format: Whole IgG
  • Applications
    Western Blot, ImmunoHistoChemistry
  • Applications Supplier
    WB - 1:2,000 - 1:10,000; IP - Not recommended. Use rabbit anti-SUPT5H antibody A300-868A.; IHC - 1:500 - 1:2,000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections. IHC, WB
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1000 ug/ml
  • Conjugate
    Unconjugated
  • Gene ID6829
  • Target name
    SUPT5H
  • Target description
    SPT5 homolog, DSIF elongation factor subunit
  • Target synonyms
    SPT5, SPT5H, Tat-CT1, transcription elongation factor SPT5, DRB sensitivity-inducing factor 160 kDa subunit, DRB sensitivity-inducing factor large subunit, DSIF large subunit, DSIF p160, Tat-cotransactivator 1 protein, hSPT5, suppressor of Ty 5 homolog
  • Host
    Rabbit
  • Protein IDO00267
  • Protein Name
    Transcription elongation factor SPT5
  • Scientific Description
    SUPT5H Antibody
  • Storage Instruction
    2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of SPT5/SUPT5H using anti-SPT5/SUPT5H antibody (A03426-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: mouse RAW264.7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SPT5/SUPT5H antigen affinity purified polyclonal antibody (Catalog # A03426-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SPT5/SUPT5H at approximately 150 kDa. The expected band size for SPT5/SUPT5H is at 121 kDa.
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