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Rabbit anti-TFAM Antibody, Affinity Purified

A303-225A
Bethyl Laboratories
ApplicationsImmunoPrecipitation, ImmunoHistoChemistry
Product group Antibodies
TargetTFAM
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Overview

  • Supplier
    Bethyl Laboratories
  • Product Name
    Rabbit anti-TFAM Antibody, Affinity Purified
  • Delivery Days Customer
    9
  • Applications
    ImmunoPrecipitation, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1000 ug/ml
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID7019
  • Target name
    TFAM
  • Target description
    transcription factor A, mitochondrial
  • Target synonyms
    MTDPS15, MTTF1, MTTFA, TCF6, TCF6L1, TCF6L2, TCF6L3, transcription factor A, mitochondrial, mitochondrial transcription factor 1, mitochondrial transcription factor A, transcription factor 6, transcription factor 6-like 1, transcription factor 6-like 2 (mitochondrial transcription factor), transcription factor 6-like 3
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ00059
  • Protein Name
    Transcription factor A, mitochondrial
  • Storage Instruction
    2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of mtTFA using anti-mtTFA antibody (PB9447). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: Caco-2 whole cell lysates, Lane 2: SW620 whole cell lysates, Lane 3: PANC-1 whole cell lysates, Lane 4: rat brain tissue lysates, Lane 5: mouse brain tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-mtTFA antigen affinity purified polyclonal antibody (Catalog # PB9447) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for mtTFA at approximately 24KD. The expected band size for mtTFA is at 24KD.
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