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ProductsBack/Rabbit anti-TIF1 Alpha/TRIM24 Antibody, Affinity Purified

Rabbit anti-TIF1 Alpha/TRIM24 Antibody, Affinity Purified

A300-815A
Bethyl Laboratories
ApplicationsImmunoPrecipitation, Western Blot, ChIP Chromatin ImmunoPrecipitation, ImmunoHistoChemistry
Product group Antibodies
TargetTRIM24
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Overview

  • Supplier
    Bethyl Laboratories
  • Product Name
    Rabbit anti-TIF1 Alpha/TRIM24 Antibody, Affinity Purified
  • Delivery Days Customer
    9
  • Applications
    ImmunoPrecipitation, Western Blot, ChIP Chromatin ImmunoPrecipitation, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    200 ug/ml
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID8805
  • Target name
    TRIM24
  • Target description
    tripartite motif containing 24
  • Target synonyms
    PTC6, RNF82, TF1A, TIF1, TIF1A, TIF1ALPHA, hTIF1, transcription intermediary factor 1-alpha, E3 ubiquitin-protein ligase TRIM24, RING finger protein 82, RING-type E3 ubiquitin transferase TIF1-alpha, TIF1-alpha, transcriptional intermediary factor 1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDO15164
  • Protein Name
    Transcription intermediary factor 1-alpha
  • Storage Instruction
    2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of TRIM24 using anti-TRIM24 antibody (A03258-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human MCF-7 whole cell lysates, Lane 3: human SW620 whole cell lysates, Lane 4: human HepG2 whole cell lysates, Lane 5: rat RH35 whole cell lysates, Lane 6: mouse HEPA1-6 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TRIM24 antigen affinity purified polyclonal antibody (Catalog # A03258-3) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TRIM24 at approximately 130KD. The expected band size for TRIM24 is at 117KD.
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