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Rabbit anti-TRPM7 Antibody, Affinity Purified

A302-700A
Bethyl Laboratories
ApplicationsImmunoPrecipitation, Western Blot
Product group Antibodies
ReactivityHuman, Mouse
TargetTRPM7

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Overview

  • Supplier
    Bethyl Laboratories
  • Product Name
    Rabbit anti-TRPM7 Antibody, Affinity Purified
  • Delivery Days Customer
    9
  • Applications
    ImmunoPrecipitation, Western Blot
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    200 ug/ml
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID54822
  • Target name
    TRPM7
  • Target description
    transient receptor potential cation channel subfamily M member 7
  • Target synonyms
    ALSPDC, CHAK, CHAK1, LTRPC7, LTrpC-7, TRP-PLIK, transient receptor potential cation channel subfamily M member 7, LTRPC ion channel family member 7, channel-kinase 1, long transient receptor potential channel 7, transient receptor potential-phospholipase C-interacting kinase
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ96QT4
  • Protein Name
    Transient receptor potential cation channel subfamily M member 7
  • Reactivity
    Human, Mouse
  • Storage Instruction
    2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of TRPM7 using anti-TRPM7 antibody (A00789-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Hela whole cell lysate, Lane 2: human COLO-320 whole cell lysate, Lane 3: human 22RV1 whole cell lysate, Lane 4: human SGC-7901 whole cell lysate. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TRPM7 antigen affinity purified polyclonal antibody (Catalog # A00789-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TRPM7 at approximately 250KD. The expected band size for TRPM7 is at 212KD.
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