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Rabbit anti-UBA5 Antibody, Affinity Purified

A304-114A
Bethyl Laboratories
ApplicationsImmunoPrecipitation
Product group Antibodies
TargetUBA5
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Overview

  • Supplier
    Bethyl Laboratories
  • Product Name
    Rabbit anti-UBA5 Antibody, Affinity Purified
  • Delivery Days Customer
    9
  • Applications
    ImmunoPrecipitation
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1000 ug/ml
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID79876
  • Target name
    UBA5
  • Target description
    ubiquitin like modifier activating enzyme 5
  • Target synonyms
    DEE44, EIEE44, SCAR24, THIFP1, UBE1DC1, ubiquitin-like modifier-activating enzyme 5, UBA5, ubiquitin-activating enzyme E1 homolog, UFM1-activating enzyme, ubiquitin-activating enzyme 5, ubiquitin-activating enzyme E1 domain-containing protein 1, ubiquitin-activating enzyme E1-domain containing 1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9GZZ9
  • Protein Name
    Ubiquitin-like modifier-activating enzyme 5
  • Storage Instruction
    2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of UBA5 using anti-UBA5 antibody (A06385-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human CACO-2 whole cell lysates, Lane 2: human Jurkat whole cell lysates, Lane 3: human Hela whole cell lysates, Lane 4: rat pancreas tissue lysates, Lane 5: mouse pamcreas tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-UBA5 antigen affinity purified polyclonal antibody (Catalog # A06385-2) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for UBA5 at approximately 50 kDa. The expected band size for UBA5 is at 45 kDa.
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