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ProductsBack/Rabbit anti-USP5/IsoT Antibody, Affinity Purified

Rabbit anti-USP5/IsoT Antibody, Affinity Purified

A301-542A
Bethyl Laboratories
ApplicationsImmunoPrecipitation, Western Blot
Product group Antibodies
TargetUSP5
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Overview

  • Supplier
    Bethyl Laboratories
  • Product Name
    Rabbit anti-USP5/IsoT Antibody, Affinity Purified
  • Delivery Days Customer
    9
  • Applications
    ImmunoPrecipitation, Western Blot
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    200 ug/ml
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID8078
  • Target name
    USP5
  • Target description
    ubiquitin specific peptidase 5
  • Target synonyms
    ISOT, ubiquitin carboxyl-terminal hydrolase 5, deubiquitinating enzyme 5, isopeptidase T, testicular tissue protein Li 218, ubiquitin isopeptidase T, ubiquitin specific protease 5 (isopeptidase T), ubiquitin thioesterase 5, ubiquitin thiolesterase 5, ubiquitin-specific protease-5 (ubiquitin isopeptidase T), ubiquitin-specific-processing protease 5
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP45974
  • Protein Name
    Ubiquitin carboxyl-terminal hydrolase 5
  • Storage Instruction
    2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of USP5 using anti-USP5 antibody (A04550-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human A549 whole cell lysates, Lane 2: human U20S whole cell lysates, Lane 3: human MDA-MB-453 whole cell lysates, Lane 4: huamn MCF-7 whole cell lysates, Lane 5: rat brain tissue lysates, Lane 6: rat testis tissue lysates, Lane 7: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-USP5 antigen affinity purified polyclonal antibody (Catalog # A04550-1) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for USP5 at approximately 96 kDa. The expected band size for USP5 is at 96 kDa.
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