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ProductsBack/RACK1 Rabbit Polyclonal Antibody
Gel: 12%SDS-PAGE Lysate: 50 microg Lane 1-2: NIH/3T3 cells Jurkat cells Primary antibody: TA364954 (RACK1 Antibody) at dilution 1/300 Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution Exposure time: 20 seconds
Gel: 12%SDS-PAGE Lysate: 50 microg Lane 1-2: NIH/3T3 cells Jurkat cells Primary antibody: TA364954 (RACK1 Antibody) at dilution 1/300 Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution Exposure time: 20 seconds
Gel: 12%SDS-PAGE Lysate: 50 microg Lane 1-2: NIH/3T3 cells Jurkat cells Primary antibody: TA364954 (RACK1 Antibody) at dilution 1/300 Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution Exposure time: 20 seconds

RACK1 Rabbit Polyclonal Antibody

TA364954
OriGene
ApplicationsWestern Blot
DownloadMSDS
Product group Antibodies
TargetRACK1
100 ul
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Overview

  • Supplier
    OriGene
  • Product Name
    RACK1 Rabbit Polyclonal Antibody
  • Delivery Days Customer
    14
  • Applications
    Western Blot
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Gene ID10399
  • Target name
    RACK1
  • Target description
    receptor for activated C kinase 1
  • Target synonyms
    cell proliferation-inducing gene 21 protein; GNB2L1; Gnb2-rs1; guanine nucleotide binding protein (G protein), beta polypeptide 2-like 1; guanine nucleotide binding protein beta polypeptide 2-like 1; guanine nucleotide-binding protein subunit beta-2-like 1; guanine nucleotide-binding protein subunit beta-like protein 12.3; H12.3; HLC-7; human lung cancer oncogene 7 protein; lung cancer oncogene 7; PIG21; proliferation-inducing gene 21; protein homologous to chicken B complex protein, guanine nucleotide binding; receptor of activated protein C kinase 1; receptor of activated protein kinase C 1; small ribosomal subunit protein RACK1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP63244
  • Protein Name
    Receptor of activated protein C kinase 1
  • Scientific Description
    RACK1 rabbit polyclonal antibody
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of RACK1 using anti-RACK1 antibody (PB9243). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human MCF-7 whole cell lysates, Lane 2: human Jurkat whole cell lysates, Lane 3: human HepG2 whole cell lysates, Lane 4: human A549 whole cell lysates, Lane 5: human Raji whole cell lysates, Lane 6: rat liver tissue lysates, Lane 7: mouse RAW264.7 whole cell lysates, Lane 8: mouse HEPA1-6 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RACK1 antigen affinity purified polyclonal antibody (Catalog # PB9243) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RACK1 at approximately 36 kDa and for RACK1 mature form at approximately 32 kDa. The expected band size for RACK1 is at 35 kDa.
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