Non-transfected (–) and transfected (+) HeLa whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with Rad50 antibody [N1N2], N-term (GTX119731) diluted at 1:4000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
![Rad50 antibody [N1N2], N-term detects RAD50 protein by western blot analysis. A. 30 μg Neuro2A whole lysate/extract B. 30 μg GL261 whole cell lysate/extract 5% SDS-PAGE Rad50 antibody [N1N2], N-term (GTX119731) dilution: 1:1000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX119731/GTX119731_40344_WB_M_w_23060519_834.webp "Rad50 antibody [N1N2], N-term detects RAD50 protein by western blot analysis. A. 30 μg Neuro2A whole lysate/extract B. 30 μg GL261 whole cell lysate/extract 5% SDS-PAGE Rad50 antibody [N1N2], N-term (GTX119731) dilution: 1:1000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.")
![Whole cell extract (30 μg) was separated by 5% SDS-PAGE, and the membrane was blotted with Rad50 antibody [N1N2], N-term (GTX119731) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX119731/GTX119731_40344_20160811_WB_R_w_23060519_756.webp "Whole cell extract (30 μg) was separated by 5% SDS-PAGE, and the membrane was blotted with Rad50 antibody [N1N2], N-term (GTX119731) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.")
![HeLa whole cell and nuclear extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with Rad50 antibody [N1N2], N-term (GTX119731) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX119731/GTX119731_40344_20151119_WB_Fraction_w_23060519_567.webp "HeLa whole cell and nuclear extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with Rad50 antibody [N1N2], N-term (GTX119731) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.")
![Immunoprecipitation of Rad50 protein from NT2D1 whole cell extract using 5 μg of Rad50 antibody [N1N2], N-term (GTX119731). Western blot analysis was performed using Rad50 antibody [N1N2], N-term (GTX119731). EasyBlot HRP-conjugated anti rabbit IgG antibody (GTX221666-01).](https://www.genetex.com/upload/website/prouct_img/normal/GTX119731/GTX119731_40344_20240419_IP_24042400_203.webp "Immunoprecipitation of Rad50 protein from NT2D1 whole cell extract using 5 μg of Rad50 antibody [N1N2], N-term (GTX119731). Western blot analysis was performed using Rad50 antibody [N1N2], N-term (GTX119731). EasyBlot HRP-conjugated anti rabbit IgG antibody (GTX221666-01).")
Non-transfected (–) and transfected (+) HeLa whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with Rad50 antibody [N1N2], N-term (GTX119731) diluted at 1:4000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Rad50 antibody [N1N2], N-term
GTX119731
ApplicationsImmunoPrecipitation, Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetRAD50
Overview
- SupplierGeneTex
- Product NameRad50 antibody [N1N2], N-term
- Delivery Days Customer9
- Application Supplier NoteWB: 1:500-1:10000. IHC-P: 1:100-1:1000. IP: 1:100-1:500. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsImmunoPrecipitation, Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration0.78 mg/ml
- ConjugateUnconjugated
- Gene ID10111
- Target nameRAD50
- Target descriptionRAD50 double strand break repair protein
- Target synonymsNBSLD, RAD502, hRad50, DNA repair protein RAD50, RAD50 homolog, double strand break repair protein
- HostRabbit
- IsotypeIgG
- Protein IDQ92878
- Protein NameDNA repair protein RAD50
- Scientific DescriptionThe protein encoded by this gene is highly similar to Saccharomyces cerevisiae Rad50, a protein involved in DNA double-strand break repair. This protein forms a complex with MRE11 and NBS1. The protein complex binds to DNA and displays numerous enzymatic activities that are required for nonhomologous joining of DNA ends. This protein, cooperating with its partners, is important for DNA double-strand break repair, cell cycle checkpoint activation, telomere maintenance, and meiotic recombination. Knockout studies of the mouse homolog suggest this gene is essential for cell growth and viability. Two alternatively spliced transcript variants of this gene, which encode distinct proteins, have been reported. [provided by RefSeq]
- ReactivityHuman, Mouse, Rat
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203
References
- Heiser K, Nicholas C, Garcea RL. Activation of DNA damage repair pathways by murine polyomavirus. Virology. 2016,497:346-356. doi: 10.1016/j.virol.2016.07.028Read this paper
Datasheet
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